{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/2051996266904739072.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"NAID","@value":"110002106313"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/digimeta/10693524"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/10693524"}},{"identifier":{"@type":"URI","@value":"http://dl.ndl.go.jp/info:ndljp/pid/10693524"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/1993019329"}}],"dc:title":[{"@value":"ヒト乳癌細胞株MCF-7細胞に対するaromatase阻害剤の増殖抑制効果"}],"dcterms:alternative":[{"@value":"ヒト乳癌細胞株MCF-7細胞に対するaromatase阻害剤の増殖抑制効果"},{"@value":"Growth Inhibition of MCF-7 Human Breast Cancer Cells by Aromatase Inhibitors"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@value":"estrogen依存性腫瘍である乳癌, 子宮内膜癌などに対する抗腫瘍薬物療法としてestrogen生合成酵素であるaromataseの阻害剤が有用と考えられ, 乳癌では以前よりaminoglutethimide (AG) が欧米で使用されている。しかしAGは他のcholesterol系の酵素も阻害するためaromataseに選択的な副作用の少ない他の薬剤の開発, 臨床治験がすすめられている。閉経期の腫瘍増殖におけるaromataseの作用機序としては末梢の組織におけるestrogenの生成に寄与していること以外あまり知られていない。そこで今回estrogen receptor (ER) 及びaromatase活性を有するヒト乳癌培養樹立細胞株MCF-7をモデルとして用い, 腫瘍細胞の細胞増殖におけるaromataseの関与並びにaromatase阻害剤の効果につき検討した。細胞増殖は〔^3H〕thymidineの取り込みにより細胞のDNA合成能を通して測定した。生理的濃度のestradiol, estrone, testosterone (T) 及びandrostenedione (Δ_4A) により, DNA合成能は増加したが, estrone sulfate, dihydrotestosterone (DHT) では生理的濃度より高濃度でのみ増加がみられた。T及びDHTによるDNA合成能の増加はtamoxifenにより抑制されたが, androgen receptor (AR) の阻害剤であるcyproterone acetate (CPA) による抑制は受けなかった。このことはT及びDHTによる細胞増殖がARではなくERを介した効果発現と考えられた。またT及びΔ_4AによるDNA合成能の増加はaromatase阻害剤であるaminoglutethimide (AG) 及び14α-hydroxy-4-androstene-3,6,17-trione (14α-OHAT) により抑制を受けたが, nonaromatizable androgenであるDHTによるDNA合成能の増加はaromatase阻害剤による抑制を受けなかった。つまりandrogenが腫瘍細胞内のaromataseによりestrogenに変換されMCF-7の腫瘍増殖に部分的に寄与していることが明らかとなった。また腫瘍増殖抑制にaromatase阻害剤の有用性が示唆された。"},{"@value":"MCF-7 cell line is a model for estrogen-dependent tumors that have both aromatase activity and estrogen receptor. We studied the contribution of aromatase to cell growth and DNA synthesis by means of aromatase inhibitors. MCF-7 cells were cultured in phenol red-free medium containing 10% charcoal-treated fetal bovine serum and test reagents for 96 hours and pulse-labeled with [^3H]thymidine for 1 hour. Physiological concentrations of estradiol, estrone, testosterone (T) and androstenedione (Δ_4A) increased [3H]thymidine incorporation. Stimulation by T or dihydrotestosterone (DHT) was reduced by tamoxifen, but not by androgen receptor blocker cyproterone acetate, suggesting that T and DHT stimulated cellular proliferation via estrogen receptor but not via androgen receptor. Stimulation by T or Δ_4A was reduced by aromatase inhibitors (aminoglutethimide and 14α-hydroxy-4-androstene-3,6,17-trione), but stimulation by nonaromatizable DHT was not reduced by aromatase inhibitors. These results have suggested that estrogens which are biosynthesized from androgens by the intracellular aromatase play a significant role in growth stimulation of MCF-7 cells and that aromatase inhibitors block this pathway. These methods are useful in assessing the ability of aromatase inhibitors to suppress cell growth."}]},{"notation":[{"@value":"収集根拠 : NII-ELS"},{"@value":"資料形態 : テキストデータ"},{"@value":"コレクション : 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会"},{"@value":"著者所属: 京都府立医科大学産婦人科学教室"},{"@value":"Affiliation: Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1580009750156152577","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000364234930"}],"foaf:name":[{"@value":"福岡,正晃"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003825724502786","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00204925"},{"@type":"NRID","@value":"1000000204925"},{"@type":"NRID","@value":"9000364578283"},{"@type":"NRID","@value":"9000364577425"},{"@type":"NRID","@value":"9000364581124"},{"@type":"NRID","@value":"9000364580131"},{"@type":"NRID","@value":"9000364076519"},{"@type":"NRID","@value":"9000364582963"},{"@type":"NRID","@value":"9000364582652"},{"@type":"NRID","@value":"9000364584724"},{"@type":"NRID","@value":"9000364585607"},{"@type":"NRID","@value":"9000364585169"},{"@type":"NRID","@value":"9000004711005"},{"@type":"NRID","@value":"9000364445966"},{"@type":"NRID","@value":"9000364577787"},{"@type":"NRID","@value":"9000364348566"},{"@type":"NRID","@value":"9000364331795"},{"@type":"NRID","@value":"9000364299814"},{"@type":"NRID","@value":"9000364200948"},{"@type":"NRID","@value":"9000364371958"}],"foaf:name":[{"@value":"北脇,城"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658324088576","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60158285"},{"@type":"NRID","@value":"1000060158285"},{"@type":"NRID","@value":"9000364234356"},{"@type":"NRID","@value":"9000364076518"},{"@type":"NRID","@value":"9000021843614"},{"@type":"NRID","@value":"9000364285260"},{"@type":"NRID","@value":"9000364200952"},{"@type":"NRID","@value":"9000364270132"},{"@type":"NRID","@value":"9000364212026"}],"foaf:name":[{"@value":"山本,宝"}]},{"@id":"https://cir.nii.ac.jp/crid/1581698602132604675","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000364152083"}],"foaf:name":[{"@value":"岡田,弘二"}]}],"publication":{"publicationIdentifier":[{"@type":"NCID","@value":"AN00190060"}],"prism:publicationName":[{"@value":"日本産科婦人科學會雜誌"}],"dc:publisher":[{"@value":"日本産科婦人科学会"}],"prism:publicationDate":"1991-12-01","prism:volume":"43(12)","prism:startingPage":"1667","prism:endingPage":"1673"},"url":[{"@id":"http://id.ndl.go.jp/digimeta/10693524"},{"@id":"https://dl.ndl.go.jp/pid/10693524"},{"@id":"http://dl.ndl.go.jp/info:ndljp/pid/10693524"},{"@id":"https://search.jamas.or.jp/link/ui/1993019329"}],"foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Aromatase","dc:title":"Aromatase"},{"@id":"https://cir.nii.ac.jp/all?q=MCF-7","dc:title":"MCF-7"},{"@id":"https://cir.nii.ac.jp/all?q=Breast%20cancer","dc:title":"Breast cancer"},{"@id":"https://cir.nii.ac.jp/all?q=Thymidine%20incorporation","dc:title":"Thymidine incorporation"},{"@id":"https://cir.nii.ac.jp/all?q=Estrogen%20receptor","dc:title":"Estrogen receptor"}],"dataSourceIdentifier":[{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R100000039-I10693524"},{"@type":"NDL_DC","@value":"info:ndljp/pid/10693524"},{"@type":"CIA","@value":"110002106313"}]}