[Updated on July 12] Integration of CiNii Articles into CiNii Research from April 1, 2022

Real-time RT-PCR detection method for SARS-CoV-2

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Abstract

type:Regular Article

Emerging in December, 2019, SARS-CoV-2 is spreading worldwide, endangering the citizens of the globe. The authors sought to develop a feasible real-time RT-PCR method to detect RNA of the virus. The fluorescent probe the authors designed proved its potency and utility, by detecting in vitro transcribed RNA sequence at an estimated concentration approximating a single copy per reaction, used along with 3 sets of primers that flank the probe sequence. Though not tested against actual viral RNA, or clinical specimens, this method can serve as another choice of rapid detection of the plague-causing virus.

source:https://igakkai.kms-igakkai.com/wp/wp-content/uploads/2020en/KMJ-E202046097.pdf

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