Promotion of direct reprogramming by transformation-deficient Myc
Induced pluripotent stem cells (iPSCs) are generated from mouse and human fibroblasts by the introduction of three transcription factors: Oct3/4, Sox2, and Klf4. The proto-oncogene product c-Myc markedly promotes iPSC generation, but also increases tumor formation in iPSC-derived chimeric mice. We report that the promotion of iPSC generation by Myc is independent of its transformation property. We found that another Myc family member, L-Myc, as well as c-Myc mutants (W136E and dN2), all of which have little transformation activity, promoted human iPSC generation more efficiently and specifically compared with WT c-Myc. In mice, L-Myc promoted germline transmission, but not tumor formation, in the iPSC-derived chimeric mice. These data demonstrate that different functional moieties of the Myc proto-oncogene products are involved in the transformation and promotion of directed reprogramming.
形質転換活性を欠損したMycによるリプログラミング促進効果：L-Mycを用いた効率的なiPS細胞の樹立. 京都大学プレスリリース. 2010-07-27.
- Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences 107 (32), 14152-14157, 2010-07-26
The National Academy of Sciences