- IN VITRO CHARACTERIZATION OF HEAT-TREATED ACTIVATED PROTHROMBIN COMPLEX CONCENTRATES (APCCs)
We studied in vitro properties of heat-treated APCCs (Autoplex T and FEIBA TIM4) as compared with a heat-treated PCC (Proplex ST) with special reference to vitamin K dependent factors and F. Ⅷ inhibitor bypassing activity (FEIB-activity). Autoplex and FEIBA were found to contain all of Factors Ⅱ, Ⅶ, Ⅸ and Ⅹ activities equal to or more than those levels in Proplex. While the immunological activity of F. Ⅱ and F. Ⅹ in both APCCs was higher than their functional activity, the functional activity of F. Ⅶ and F. Ⅸ was higher than their immunological activity. With western blot technique, bands of F. Ⅶa, F. Ⅸa and F. Ⅹa were shown in autoplex, FEIBA and Proplex. The content of FEIB-activity in both APCCs was the same as or more than indicated individually on the label. These findings suggest that activation and inactivation of biological activity and some modification of prothrombin family proteins in Autoplex and FEIBA occurred during production and heating procedures, and that both APCCs are probably effective and useful for managing hemophiliacs with inhibitors.
identifier:奈良医学雑誌 Vol.41 No.5 p.547-554
identifier:奈良医学雑誌, 41(5): 547-554
奈良医学雑誌 41 (5), 547-554, 1990-10-31