Emerging in December, 2019, SARS-CoV-2 is spreading worldwide, endangering the citizens of the globe. The authors sought to develop a feasible real-time RT-PCR method to detect RNA of the virus. The fluorescent probe the authors designed proved its potency and utility, by detecting in vitro transcribed RNA sequence at an estimated concentration approximating a single copy per reaction, used along with 3 sets of primers that flank the probe sequence. Though not tested against actual viral RNA, or clinical specimens, this method can serve as another choice of rapid detection of the plague-causing virus.
Kawasaki medical journal 46 97-101, 2020
Kawasaki Medical Society