Chromatographic Separation Property of Disaccharides with a Cation-Exchange Resin
type:Departmental Bulletin Paper
It was attempted to estimate the binding constant, B, of a disaccharide, to a counter-ion from the apparent distribution coefficient, Kapp, of the solute to the resin with DVB content of 8％ in cation-exchago chromatography. The Kapp values for heterogeneous disaccharides were higher than those for homogeneous disacchuarides except maltose. The B values for disaccharides were estimated under the assumption that the binding constant of gentiobiose, which has the lowest Kapp value in disaccharides used, is zero: that is, no complex is formed between gontiobiose and Na^＋. It was indicated that the ability of heterogeneous disaccharide for the complex formation would be high in the resin phase because of its high B value.
- 近畿大学工学部研究報告 = Research reports of the School of Engineering, Kinki University
近畿大学工学部研究報告 = Research reports of the School of Engineering, Kinki University 42 21-25, 2008-12-01