- Preparation of Bone Morphogenetic Protein and its Bioactivity
In the present investigation, BMP (bone morphogenetic protein) was obtained according to the methods of Urist et al. (1984). The bovine demineralized bone matrix was extracted with 6M Urea and the supernatant solution was dialyzed against water. The precipitate was collected by centrifugation (Urea Fraction-1: UF-1). The UF-1 was redissolved in 6M urea solution and dialyzed against a citrate buffer (pH 3.1). After that, a grayish-white precipitate was collected (UF-2). Histopathological study revealed that both UF-1 and UF-2 induced subcutaneous and perimusclar tissues of rats to form bone, indicating that UF-1 and UF-2 have BMP activities. Furthermore, suqualane-UF complex elicited heterotopic formation of wide bone with bone marrow tissue.
松本歯学 18 (3), 244-249, 1992-12-31