- ELECTROPHORETIC ANALYSIS OF URINARY GLYCOPROTEIN FOR DIABETIC NEPHROPATHY USING PEROXIDASE-LECTINS
To evaluate the severity of diabetic renal involvement, we performed electrophoretic analysis of urinary glycoprotein for 16 patients with diabetic nephropathy using peroxidase-lectins. After being subjected to SDS-polyacrylamide gel electrophoresis, urinary glycoprotein was transferred to the nitrocellulose membrane, which then was stained with peroxidase labeled-lectin (Triticum vulgaris ; WGA), and was analyzed densitometrically. Electrophoretic analysis of urinary glycoprotein revealed the presence of lectin-reactive glycoproteins with the molecular weights of 22 kD, 32 kD, 38 kD and 48 kD respectively. Among these, the amount of the 22 kD-mol. wt. glycoprotein increased significantly as the renal histopathological changes developed, whereas that of 32 kD decreased. These results suggest that semiquantitative analysis of low molecular weight urinary glycoprotein stained with lectin is useful for the non-invasive evaluation of the severity of diabetic nephropathy.
identifier:奈良医学雑誌 Vol.43 No.6 p.598-603
identifier:奈良医学雑誌, 43(6): 598-603
奈良医学雑誌 43 (6), 598-603, 1992-12-31