CiNii Researchの本公開について

糖尿病性腎症における尿中レクチン結合性低分子糖蛋白分画

書誌事項

タイトル別名
  • ELECTROPHORETIC ANALYSIS OF URINARY GLYCOPROTEIN FOR DIABETIC NEPHROPATHY USING PEROXIDASE-LECTINS

この論文をさがす

抄録

type:Article

To evaluate the severity of diabetic renal involvement, we performed electrophoretic analysis of urinary glycoprotein for 16 patients with diabetic nephropathy using peroxidase-lectins. After being subjected to SDS-polyacrylamide gel electrophoresis, urinary glycoprotein was transferred to the nitrocellulose membrane, which then was stained with peroxidase labeled-lectin (Triticum vulgaris ; WGA), and was analyzed densitometrically. Electrophoretic analysis of urinary glycoprotein revealed the presence of lectin-reactive glycoproteins with the molecular weights of 22 kD, 32 kD, 38 kD and 48 kD respectively. Among these, the amount of the 22 kD-mol. wt. glycoprotein increased significantly as the renal histopathological changes developed, whereas that of 32 kD decreased. These results suggest that semiquantitative analysis of low molecular weight urinary glycoprotein stained with lectin is useful for the non-invasive evaluation of the severity of diabetic nephropathy.

identifier:奈良医学雑誌 Vol.43 No.6 p.598-603

identifier:04695550

identifier:13450069

identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1854

identifier:奈良医学雑誌, 43(6): 598-603

収録刊行物

被引用文献 (0)

もっと見る

参考文献 (0)

もっと見る

関連論文

もっと見る

関連研究データ

もっと見る

関連図書・雑誌

もっと見る

関連博士論文

もっと見る

関連プロジェクト

もっと見る

関連その他成果物

もっと見る

詳細情報

ページトップへ