- TISSUE RECONSTRUCTION OF HUMAN NORMAL AND TUMOR CELLS MAINLY FROM THE HEAD AND NECK REGION USING THREE-DIMENSIONAL CULTURE
Three-dimensional culture was performed using gamma-irradiated type Ⅰ collagen gel prepared from rat tail tendon. The morphological outgrowing appearance with in type Ⅰ collagen gel provided a marked difference in accordance with histopathological diagnosis, subsequently suggesting the difference in adhesion ability to type Ⅰ collagen. On the other hand, type Ⅰ collagen may be involved in differentiation-related proteins expressed during in vitro morphogenesis, such as carcinoembryonic antigen for tubulogenesis of the salivary gland. The present experiment system would be useful for studying further the relation between type Ⅰ collagen matrix and metastatic potentiality and/or cellular differentiation.
identifier:奈良医学雑誌 Vol.43 No.4 p.260-271
identifier:奈良医学雑誌, 43(4): 260-271
奈良医学雑誌 43 (4), 260-271, 1992-08-31