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へムたんぱく-ガス状リガンド結合の反応速度論 : Ⅱ. グリコシル化微少ヘモグロビン,Hb Aɪс,Aɪь,Aɪa₂,Aɪa₁,およびHb Hope,β136(H 14)Gly→Asp,について

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  • KINETICS OF THE REACTIONS OF HEME PROTEINS WITH GASEOUS LIGANDS : STUDIES WITH STOPPED-FLOW SPECTROPHOTOMETRY : Ⅱ. THE REACTION OF HUMAN GLYCOSYLATED MINOR HEMOGLOBINS, HB Aɪс, Aɪь, Aɪa₂, Aɪa₁, AND HB HOPE, β136(H 14)Gly→Asp, WITH OXYGEN AND CARBON MONOXIDE

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type:Article

The kinetics of the ligand binding to the glycosylated minor components of human adult hemoglobin (Hb Aɪс Aɪь, Aɪa₂ and Aɪa₁) and a variant hemoglobin, Hb Hope, were studied with the stopped-flow method. The rate constants of O₂ dissociation and CO association for the minor hemoglobins were less affected by 2, 3-diphosphoglycerate (DPG) or inositol hexaphosphate (IHP) than for the major component, Hb A₀. The rate constants for Hb Aɪa₁ and Aɪa₂ were practically unaffected by these organic phosphates. As for Hb Hope, the effects of DPG and IHP were reduced on the CO association rate constant (l'), but not on the O₂ dissociation rate constant (k). These results were consistent with the O₂ equilibrium findings. Hb Aɪa₁ and Aɪa₂, and Hb Hope exhibited biphasic O₂ dissociation kinetic profiles. The rate constants for the slow phase were in good agreement with that of O₂ dissociation from the isolated α chain of normal human adult hemoglobin (Hb A₀). The rate constant of O₂ dissociation from the isolated β chain of Hb Hope was approximately 4 times larger than that from the β chain of Hb A₀. It is highly probable that the biphasic O₂ dissociation kinetics reflect the large difference in reactivity to the ligand between α and β chains. I discuss the structure-function relationship of these β-variant hemoglobins from a kinetic standpoint.

identifier:奈良医学雑誌 Vol.43 No.4 p.341-353

identifier:04695550

identifier:13450069

identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1890

identifier:奈良医学雑誌, 43(4): 341-353

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