p53 Immunohistochemistry of Gallbladder Carcinoma : A Comparison of PAb1801 and DO7 Antibodies
p53 gene mutations are common in many human cancers. Many investigators have examined cancer tissue by immunnohistochemistry using commercially available p53 antibodies. However, there have been only a few reports comparing different p53 antibodies. In this study, we have examined the distribution pattern, staining intensity and index of p53-positive cells with D07 (DO-7) and PAb1801 (Ab-2) antibodies, using a 3- or 6-min diaminobenzidine (DAB) reaction time in 21 cases of gallbladder carcinoma to clarify which antibody is better for p53 detection. The distribution pattern of p53-positive cells was similar for both PAb1801 and DO7 in all but two cases. In staining with PAb1801, the p53-positive cell index was significantly higher at a 6-min reaction time than a 3-min reaction time (69.5±12.4% vs 85.1%±5.9%, p<0.01). In staining with DO7, the index was similar between 3-min and 6-min reaction times. There was no difference in the index between PAb1801 and DO7 for a 6-min reaction time (79.3±7.6% vs 85.1±5.9%). The staining intensity was stronger for 6 min than 3 min for both DO7 and PAb1801. Staining intensity at 6-min reaction time was similar between both antibodies. In summary, although PAb1801 staining is worse than DO7 staining for a 3-min reaction time, the two were similar with a 6-min reaction time. We conclude that the usefulness of PAb1801 is similar to DO7, provided a 6-min DAB reaction is used.
- Acta medica et biologica = Acta medica et biologica
Acta medica et biologica = Acta medica et biologica 43 (1), 53-57, 1995-03
Niigata University School of Medicine