【2022年1月締切】CiNii ArticlesへのCiNii Researchへの統合に伴う機関認証の移行確認について

【1/6更新】2022年4月1日からのCiNii ArticlesのCiNii Researchへの統合について

Preventive Enhancement of Hepatic Phase I and Phase II Enzyme in the Carcinogenesis Prevention by an Antiinflammatory Sesquiterpene α-Humulene

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Cancer chemoprevention relies on the use of naturally occurring or synthetic chemicals, known as xeno biotics, to suppress carcinogen s, through a mechanism via induction of phase I and phase II drug metabolizing enzymes. In the present study the effects of α humulene (HUM) on the expression and activities of hepa tic phase I and phase II enzymes were investigated. HUM is a sesquiterpene compound found in the essential oils of various plant species, previously suggested to be anti-inflammatory and anticarcinogenic. We first confirmed the antitumor effect of HUM by using two stage mouse skin carcinogenesis test. Skins were exposed to the tumor initiator 7,12 dimethylbenzo[a]anthracene for one week, then were treated repeatedly with HUM followed by a tumor promoter 12 O tetradecanoylphorbol 13 acetate for over 20 we eks: HUM suppressed tumor formation by 75%. We next analyzed hepatic enzymes by using the tissues from mice treated orally with HUM once a day for 4 successive days. The contents of total cytochrome P450 and cytochrome b 5 increased by up to 54% and 32% du e to HUM. The activities of hepatic phase I enzymes 7 ethoxycoumarin O deethylase, 7 ethoxyresorufin O deethylase and 7 pentoxyresorufin O depentylase increased by up to 92, 47 and 885%, respectively. As to hepatic phase II enzymes, glutathione S transfera se activities toward 1 chloro 2,4 dinitrobenzene and 1,2 dichloro 4 nitrobenzene increased by up to 67% and 178%, respectively.Moreover, HUM significantly elevated the GST activity toward potent carcinogen 4 nitroquinoline 1 oxide, NAD(P)H:quinone reduct ase activity and hepatic glutathione contents. Taken together, HUM has been demonstrated to be a bifunctional inducer, which enhances both the phase I and phase II enzymes of the liver. The present results suggest that the carcinogenesis suppression by su ch anti inflammatory or anti carcinogenic molecules as HUM involves modulations of the phase I and/or II drug metabolizing enzymes.

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詳細情報

  • CRID
    1050850623761250432
  • ISSN
    2436-2565
  • Web Site
    http://id.nii.ac.jp/1222/00000322/
  • 本文言語コード
    en
  • 資料種別
    departmental bulletin paper
  • データソース種別
    • IRDB
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