【7/12更新】2022年4月1日からのCiNii ArticlesのCiNii Researchへの統合について

Antibiofilm Effects of Azithromycin and Erythromycin on Porphyromonas gingivalis

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<jats:title>ABSTRACT</jats:title><jats:p>Antibiotic resistance of biofilm-grown bacteria contributes to chronic infections, such as marginal and periapical periodontitis, which are strongly associated with<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Porphyromonas gingivalis</jats:named-content>. Concurrent azithromycin (AZM) administration and mechanical debridement improve the clinical parameters of periodontal tissue<jats:italic>in situ</jats:italic>. We examined the<jats:italic>in vitro</jats:italic>efficacy of AZM against<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. gingivalis</jats:named-content>biofilms. The susceptibilities of adherent<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. gingivalis</jats:named-content>strains 381, HW24D1, 6/26, and W83 to AZM, erythromycin (ERY), ampicillin (AMP), ofloxacin (OFX), and gentamicin (GEN) were investigated using a static model. The optical densities of adherent<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. gingivalis</jats:named-content>cells were significantly decreased by using AZM and ERY at sub-MIC levels compared with those of the controls in all the strains tested, except for the effect of ERY on strain W83. AMP and OFX inhibited<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. gingivalis</jats:named-content>adherent cells at levels over their MICs, and GEN showed no inhibition in the static model. The effects of AZM and ERY against biofilm cells were investigated using a flow cell model. The ATP levels of<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. gingivalis</jats:named-content>biofilms were significantly decreased by AZM at concentrations below the sub-MICs; however, ERY was not effective for inhibition of<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. gingivalis</jats:named-content>biofilm cells at their sub-MICs. Furthermore, decreased density of<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. gingivalis</jats:named-content>biofilms was observed three-dimensionally with sub-MIC AZM, using confocal laser scanning microscopy. These findings suggest that AZM is effective against<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. gingivalis</jats:named-content>biofilms at sub-MIC levels and could have future clinical application for oral biofilm infections, such as chronic marginal and periapical periodontitis.</jats:p>

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