【7/12更新】2022年4月1日からのCiNii ArticlesのCiNii Researchへの統合について

Identification and characterization of Aeromonas hydrophila genes encoding the outer membrane receptor of ferrioxamine B and an AraC-type transcriptional regulator

  • Tatsuya Funahashi
    Laboratory of Hygienic Chemistry, College of Pharmaceutical Sciences, Matsuyama University, Matsuyama, Japan
  • Tomotaka Tanabe
    Laboratory of Hygienic Chemistry, College of Pharmaceutical Sciences, Matsuyama University, Matsuyama, Japan
  • Jun Maki
    Laboratory of Infectious Disease, College of Pharmaceutical Sciences, Matsuyama University, Matsuyama, Japan
  • Katsushiro Miyamoto
    Department of Microbiology, Osaka University of Pharmaceutical Sciences, Takatsuki, Japan
  • Hiroshi Tsujibo
    Department of Microbiology, Osaka University of Pharmaceutical Sciences, Takatsuki, Japan
  • Shigeo Yamamoto
    Laboratory of Hygienic Chemistry, College of Pharmaceutical Sciences, Matsuyama University, Matsuyama, Japan

抄録

<jats:title>Abstract</jats:title> <jats:p>We found that, under iron-limiting conditions, Aeromonas hydrophila ATCC 7966T could utilize the xenosiderophore desferrioxamine B (DFOB) for growth by inducing the expression of its own outer membrane receptor. Two consecutive genes, desR and desA, were selected as candidates involved in DFOB utilization. The presence of the ferric-uptake regulator boxes in their promoters suggested that these genes are under iron-dependent regulation. Mutation of desA, a gene that encodes the outer membrane receptor of ferrioxamine B, disrupted the growth of the amonabactin-deficient mutant in the presence of DFOB. β-Galactosidase reporter assays and reverse transcriptase-quantitative PCR demonstrated that desR, a gene that encodes an AraC-like regulator homolog is required for the induction of desA transcription in the presence of DFOB and under iron-limiting conditions. The functions of desA and desR were analyzed using complementation experiments. Our data provided evidence that DesA is powered primarily by the TonB2 system.</jats:p>

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