【7/12更新】2022年4月1日からのCiNii ArticlesのCiNii Researchへの統合について

In VivoDetermination of Vitamin D Function Using Transgenic Mice Carrying a Human Osteocalcin Luciferase Reporter Gene

  • Tomoko Nakanishi
    Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Nishicho 86, Tottori 683-8503, Japan
  • Rumiko Saito
    Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Nishicho 86, Tottori 683-8503, Japan
  • Makoto Taniguchi
    Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Nishicho 86, Tottori 683-8503, Japan
  • Haruka Oda
    Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Nishicho 86, Tottori 683-8503, Japan
  • Atsumi Soma
    Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Nishicho 86, Tottori 683-8503, Japan
  • Mayu Yasunaga
    Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Nishicho 86, Tottori 683-8503, Japan
  • Mariko Yamane
    Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Nishicho 86, Tottori 683-8503, Japan
  • Kenzo Sato
    Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Nishicho 86, Tottori 683-8503, Japan

抄録

<jats:p>Vitamin D is an essential factor for ossification, and its deficiency causes rickets. Osteocalcin, which is a noncollagenous protein found in bone matrix and involved in mineralization and calcium ion homeostasis, is one of the major bone morphogenetic markers and is used in the evaluation of osteoblast maturation and osteogenic activation. We established transgenic mouse line expressing luciferase under the control of a 10-kb osteocalcin enhancer/promoter sequence. Using these transgenic mice, we evaluated the active forms of vitamins D2 and D3 for their bone morphogenetic function by<jats:italic>in vivo</jats:italic>bioluminescence. As the result, strong activity for ossification was observed with 1<jats:italic>α</jats:italic>,25-hydroxyvitamin D3. Our mouse system can offer a feasible detection method for assessment of osteogenic activity in the development of functional foods and medicines by noninvasive screening.</jats:p>

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