【7/12更新】2022年4月1日からのCiNii ArticlesのCiNii Researchへの統合について

Rad51-Dependent Aberrant Chromosome Structures at Telomeres and Ribosomal DNA Activate the Spindle Assembly Checkpoint

  • Akemi Nakano
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Kenta Masuda
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Taisuke Hiromoto
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Katsunori Takahashi
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Yoshitake Matsumoto
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Ahmed G. K. Habib
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Ahmed G. G. Darwish
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Masashi Yukawa
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Eiko Tsuchiya
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
  • Masaru Ueno
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan

抄録

<jats:title>ABSTRACT</jats:title> <jats:p> The spindle assembly checkpoint (SAC) monitors defects in kinetochore-microtubule attachment or lack of tension at kinetochores and arrests cells at prometaphase. In fission yeast, the double mutant between <jats:italic>pot1</jats:italic> Δ and the helicase-dead point mutant of the RecQ helicase Rqh1 gene ( <jats:italic>rqh1-hd</jats:italic> ) accumulates Rad51-dependent recombination intermediates at telomeres and enters mitosis with those intermediates. Here, we found that SAC-dependent prometaphase arrest occurred more frequently in <jats:italic>pot1Δ rqh1-hd</jats:italic> double mutants than in <jats:italic>rqh1-hd</jats:italic> single mutants. SAC-dependent prometaphase arrest also occurred more frequently in <jats:italic>rqh1-hd</jats:italic> single mutants after cells were released from DNA replication block compared to the <jats:italic>rqh1-hd</jats:italic> single mutant in the absence of exogenous insult to the DNA. In both cases, Mad2 foci persisted longer than usual at kinetochores, suggesting a defect in kinetochore-microtubule attachment. In <jats:italic>pot1Δ rqh1-hd</jats:italic> double mutants and <jats:italic>rqh1-hd</jats:italic> single mutants released from DNA replication block, SAC-dependent prometaphase arrest was suppressed by the removal of the recombination or replication intermediates. Our results indicate that the accumulation of recombination or replication intermediates induces SAC-dependent prometaphase arrest, possibly by affecting kinetochore-microtubule attachment. </jats:p>

収録刊行物

被引用文献 (1)

もっと見る

参考文献 (48)

もっと見る

関連論文

もっと見る

関連研究データ

もっと見る

関連図書・雑誌

もっと見る

関連博士論文

もっと見る

関連プロジェクト

もっと見る

関連その他成果物

もっと見る

詳細情報

ページトップへ