【7/12更新】2022年4月1日からのCiNii ArticlesのCiNii Researchへの統合について

Dietary protein deprivation upregulates insulin signaling and inhibits gluconeogenesis in rat liver

  • Yuka Toyoshima
    Departments of1Applied Biological Chemistry2Animal SciencesGraduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan3The Chubu Institute for Advanced StudiesChubu University, Kasugai, Aichi 487-8501, Japan4Department of BioregulationNippon Medical School, Kawasaki, Kanagawa 211-8533, Japan5Organization for Interdisciplinary Research ProjectsThe University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
  • Reiko Tokita
    Departments of1Applied Biological Chemistry2Animal SciencesGraduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan3The Chubu Institute for Advanced StudiesChubu University, Kasugai, Aichi 487-8501, Japan4Department of BioregulationNippon Medical School, Kawasaki, Kanagawa 211-8533, Japan5Organization for Interdisciplinary Research ProjectsThe University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
  • Yoichiro Ohne
    Departments of1Applied Biological Chemistry2Animal SciencesGraduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan3The Chubu Institute for Advanced StudiesChubu University, Kasugai, Aichi 487-8501, Japan4Department of BioregulationNippon Medical School, Kawasaki, Kanagawa 211-8533, Japan5Organization for Interdisciplinary Research ProjectsThe University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
  • Fumihiko Hakuno
    Departments of1Applied Biological Chemistry2Animal SciencesGraduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan3The Chubu Institute for Advanced StudiesChubu University, Kasugai, Aichi 487-8501, Japan4Department of BioregulationNippon Medical School, Kawasaki, Kanagawa 211-8533, Japan5Organization for Interdisciplinary Research ProjectsThe University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
  • Tadashi Noguchi
    Departments of1Applied Biological Chemistry2Animal SciencesGraduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan3The Chubu Institute for Advanced StudiesChubu University, Kasugai, Aichi 487-8501, Japan4Department of BioregulationNippon Medical School, Kawasaki, Kanagawa 211-8533, Japan5Organization for Interdisciplinary Research ProjectsThe University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
  • Shiro Minami
    Departments of1Applied Biological Chemistry2Animal SciencesGraduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan3The Chubu Institute for Advanced StudiesChubu University, Kasugai, Aichi 487-8501, Japan4Department of BioregulationNippon Medical School, Kawasaki, Kanagawa 211-8533, Japan5Organization for Interdisciplinary Research ProjectsThe University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
  • Hisanori Kato
    Departments of1Applied Biological Chemistry2Animal SciencesGraduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan3The Chubu Institute for Advanced StudiesChubu University, Kasugai, Aichi 487-8501, Japan4Department of BioregulationNippon Medical School, Kawasaki, Kanagawa 211-8533, Japan5Organization for Interdisciplinary Research ProjectsThe University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
  • Shin-Ichiro Takahashi
    Departments of1Applied Biological Chemistry2Animal SciencesGraduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan3The Chubu Institute for Advanced StudiesChubu University, Kasugai, Aichi 487-8501, Japan4Department of BioregulationNippon Medical School, Kawasaki, Kanagawa 211-8533, Japan5Organization for Interdisciplinary Research ProjectsThe University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan

抄録

<jats:p>This study was undertaken to elucidate the effects of dietary protein deprivation on glucose metabolism and hepatic insulin signaling in rats. The results of glucose and pyruvate tolerance tests in rats fed with a 12% casein diet (12C) and a protein-free diet (PF) indicated that protein deprivation enhanced clearance of blood glucose and suppressed gluconeogenesis. Correspondingly, the mRNA level of hepatic phosphoenolpyruvate carboxykinase, a key gluconeogenic enzyme, was suppressed by dietary protein deprivation. In PF-fed rats, total tyrosine phosphorylation of insulin receptor (IR) in the liver induced by insulin injection was enhanced compared with 12C pair-fed rats due to an increase in IR protein level. In addition, protein deprivation caused an increase in protein levels of IR substrate 1 (IRS1) and IRS2, leading to the marked enhancement of insulin-induced tyrosine phosphorylation of IRS2 and its binding to the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K). Based on these results, we conclude that protein deprivation suppresses gluconeogenesis by a mechanism primarily mediated by the enhancement of the insulin signals through the IR/IRS/PI3K/mammalian target of rapamycin complex 1 pathway in the liver. Taken together with our previous report, these findings suggest that tissue-specific potentiation of insulin action in the liver and the skeletal muscle plays important roles in maintaining glucose homeostasis even when energy usage is reduced by dietary protein deprivation.</jats:p>

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