【2022年1月締切】CiNii ArticlesへのCiNii Researchへの統合に伴う機関認証の移行確認について

【1/6更新】2022年4月1日からのCiNii ArticlesのCiNii Researchへの統合について

Effects of downregulating TEAD4 transcripts by RNA interference on early development of bovine embryos

  • SAKURAI Nobuyuki
    United Graduate School of Agricultural Sciences, Iwate University, Iwate 020-8550, Japan
  • TAKAHASHI Kazuki
    United Graduate School of Agricultural Sciences, Iwate University, Iwate 020-8550, Japan
  • EMURA Natsuko
    Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan
  • HASHIZUME Tsutomu
    United Graduate School of Agricultural Sciences, Iwate University, Iwate 020-8550, Japan
  • SAWAI Ken
    United Graduate School of Agricultural Sciences, Iwate University, Iwate 020-8550, Japan

抄録

<p> Transcription factor TEA domain family transcription factor 4 (Tead4) is one of the key factors involved in the differentiation of the trophectoderm (TE) in murine embryos. However, knowledge on the roles of TEAD4 in preimplantation development during bovine embryos is currently limited. This study examined the transcript and protein expression patterns of TEAD4 and attempted to elucidate the functions of TEAD4 during bovine preimplantation development using RNA interference. TEAD4 mRNA was found to be upregulated between the 16-cell and morula stages, and nuclear localization of the TEAD4 protein was detected at the morula stage, as well as in subsequent developmental stages. TEAD4 downregulation did not affect embryonic development until the blastocyst stage, and TEAD4-downregulated embryos were capable of forming the TE under both 5% and 21% O2 conditions. Results of gene expression analysis showed that TEAD4 downregulation did not affect the expression levels of POU class 5 transcription factor 1 (OCT-4), NANOG, caudal-type homeobox 2 (CDX2), GATA binding protein 3 (GATA3), and interferon-tau (IFNT). In conclusion, TEAD4 might be dispensable for development until the blastocyst stage and TE differentiation in bovine embryos.</p>

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