説明
<jats:title>Abstract</jats:title><jats:p>In many animals, germ cell segregation occurs during early embryogenesis to protect the genome, but its origin in basal metazoans is controversial. Here, we show in the freshwater polyp<jats:italic>Hydra</jats:italic>by clonal analysis and transgenic animals that interstitial stem cells comprise two separate stem cell populations, i.e., germline and multipotent somatic stem cells. We isolated genetically labelled stem cells for a global transcriptome study and discovered a broad set of germline-specific/enriched genes including<jats:italic>Prdm9, Pax5, Dmrt1</jats:italic>. In an alternative splicing analysis, we identified many genes with germline-specific isoforms; among them, male-specific isoforms of<jats:italic>Dmrt1</jats:italic>and<jats:italic>Snf5</jats:italic>. The somatic interstitial stem cell lineage was characterized by numerous neuronal control genes like<jats:italic>Neurog</jats:italic>. But all stem cells in<jats:italic>Hydra</jats:italic>also share a core of stemness genes that has its roots in unicellular eukaryotes. This suggests an evolutionary scenario in which, at the emergence of animal multicellularity, there was an early split into a stable germline and different somatic stem cell lineages.</jats:p>
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詳細情報 詳細情報について
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- CRID
- 1360302864773217536
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- 資料種別
- preprint
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE