Singlet Oxygen Inhibits the Repair of Photosystem II by Suppressing the Translation Elongation of the D1 Protein in <i>Synechocystis</i> sp. PCC 6803

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  • Yoshitaka Nishiyama
    Cell-Free Science and Technology Research Center and Satellite Venture Business Laboratory, Ehime University, Bunkyo-cho, Matsuyama 790-8577, Japan, Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia, Department of Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan, and Department of Molecular Biomechanics, Graduate University for Advanced Studies, Myodaiji, Okazaki 444-8585, Japan
  • Suleyman I. Allakhverdiev
    Cell-Free Science and Technology Research Center and Satellite Venture Business Laboratory, Ehime University, Bunkyo-cho, Matsuyama 790-8577, Japan, Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia, Department of Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan, and Department of Molecular Biomechanics, Graduate University for Advanced Studies, Myodaiji, Okazaki 444-8585, Japan
  • Hiroshi Yamamoto
    Cell-Free Science and Technology Research Center and Satellite Venture Business Laboratory, Ehime University, Bunkyo-cho, Matsuyama 790-8577, Japan, Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia, Department of Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan, and Department of Molecular Biomechanics, Graduate University for Advanced Studies, Myodaiji, Okazaki 444-8585, Japan
  • Hidenori Hayashi
    Cell-Free Science and Technology Research Center and Satellite Venture Business Laboratory, Ehime University, Bunkyo-cho, Matsuyama 790-8577, Japan, Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia, Department of Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan, and Department of Molecular Biomechanics, Graduate University for Advanced Studies, Myodaiji, Okazaki 444-8585, Japan
  • Norio Murata
    Cell-Free Science and Technology Research Center and Satellite Venture Business Laboratory, Ehime University, Bunkyo-cho, Matsuyama 790-8577, Japan, Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia, Department of Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan, and Department of Molecular Biomechanics, Graduate University for Advanced Studies, Myodaiji, Okazaki 444-8585, Japan

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説明

Singlet oxygen, generated during photosynthesis, is a strong oxidant that can, potentially, damage various molecules of biological importance. We investigated the effects in vivo of singlet oxygen on the photodamage to photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. Increases in intracellular concentrations of singlet oxygen, caused by the presence of photosensitizers, such as rose bengal and ethyl eosin, stimulated the apparent photodamage to PSII. However, actual photodamage to PSII, as assessed in the presence of chloramphenicol, was unaffected by the production of singlet oxygen. These observations suggest that singlet oxygen produced by added photosensitizers acts by inhibiting the repair of photodamaged PSII. Labeling of proteins in vivo revealed that singlet oxygen inhibited the synthesis of proteins de novo and, in particular, the synthesis of the D1 protein. Northern blotting analysis indicated that the accumulation of psbA mRNAs, which encode the D1 protein, was unaffected by the production of singlet oxygen. Subcellular localization of polysomes with bound psbA mRNAs suggested that the primary target of singlet oxygen might be the elongation step of translation.

収録刊行物

  • Biochemistry

    Biochemistry 43 (35), 11321-11330, 2004-08-10

    American Chemical Society (ACS)

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