Diagnosis and Assessment of Monkeypox Virus (MPXV) Infection by Quantitative PCR Assay: Differentiation of Congo Basin and West African MPXV Strains

DOI Web Site Web Site 被引用文献2件
  • Saijo Masayuki
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Japan
  • Ami Yasushi
    Laboratory of Animal Experimentation, National Institute of Infectious Diseases, Japan
  • Suzaki Yuriko
    Laboratory of Animal Experimentation, National Institute of Infectious Diseases, Japan
  • Nagata Noriyo
    Laboratory of Infectious Disease Pathology, Department of Pathology, National Institute of Infectious Diseases, Japan
  • Iwata Naoko
    Laboratory of Infectious Disease Pathology, Department of Pathology, National Institute of Infectious Diseases, Japan
  • Hasegawa Hideki
    Laboratory of Infectious Disease Pathology, Department of Pathology, National Institute of Infectious Diseases, Japan
  • Ogata Momoko
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Japan
  • Fukushi Shuetsu
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Japan
  • Mizutani Tetsuya
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Japan
  • Iizuka Itoe
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Japan
  • Sakai Koji
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Japan
  • Sata Tetsutaro
    Laboratory of Infectious Disease Pathology, Department of Pathology, National Institute of Infectious Diseases, Japan
  • Kurata Takeshi
    Laboratory of Infectious Disease Pathology, Department of Pathology, National Institute of Infectious Diseases, Japan
  • Kurane Ichiro
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Japan
  • Morikawa Shigeru
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Japan

この論文をさがす

抄録

<p>Human monkeypox, an infectious disease caused by monkeypox virus (MPXV), is endemic to western and central Africa. A LightCycler quantitative PCR (LC-qPCR) system was developed for the diagnosis of this disease, targeting the A-type inclusion body gene (ATI gene) of MPXV. One naïve monkey was infected with MPXV Zr-599 (Congo Basin strain) and one with MPXV Liberia (West African strain). Another three monkeys were immunized with smallpox vaccine on 0, 3, or 7 days, respectively, before infection with MPXV Zr-599. Peripheral blood cell (PBC) and throat swab (TS) specimens were serially collected. The LC-qPCR was validated for the diagnosis of monkeypox using virus isolation. Sequencing of the partial ATI gene revealed the insertion of a unique 453-nucleotide residue in the West African strains but not in the Congo Basin strains. Specific reverse primers for Congo Basin and West African strains were designed based on the unique sequence insertion. The LC-qPCR detected the MPXV genome, but not those of the other orthopoxviruses tested nor the varicella-zoster virus. Both the sensitivity and specificity of the LC-qPCR were over 90% in comparison to virus isolation when TS specimens were tested. Fourteen of the 15 virus isolation-positive PBC specimens showed positive reactions in the assay. Further, most PBC specimens collected from symptomatic monkeys in the later stage of illness showed positive reactions in the assay but negative reaction in virus isolation. It was possible to differentiate between these two groups with the LC-qPCR. Thus, the newly developed LC-qPCR is a useful and reliable diagnostic tool for MPXV infection.<tt> </tt></p>

収録刊行物

被引用文献 (2)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ