Eriodictyol and thymonin act as GPR35 agonists

  • Fumie Nakashima
    Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan
  • Wei Qi Loh
    Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan
  • Mayuka Wakabayashi
    Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan
  • Sayako Shimomura
    Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan
  • Hiroyuki Hattori
    Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan
  • Masaki Kita
    Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan
  • Asuka Inoue
    Graduate School of Pharmaceutical Sciences, Tohoku University , Sendai , Japan
  • Koji Uchida
    Graduate School of Agricultural and Life Sciences, The University of Tokyo , Tokyo , Japan
  • Takahiro Shibata
    Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan

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<jats:title>ABSTRACT</jats:title> <jats:p>Although herbs and spices have been used in traditional medicine for more than a century owing to their health benefits, the associated underlying mechanism is still not clear. Since the G protein-coupled receptor 35 (GPR35) has been linked to exert various antioxidant and anti-inflammatory effects, we screened 19 different herbs and spices for possible GPR35 agonist(s) to understand the GPR35-dependent functions of herbs and spices. Among the screened extracts, the ethyl acetate extract of thyme exhibited a remarkable GPR35 agonistic activity. Activity-guided separations allowed us to identify 2 polyphenolic phytochemicals, eriodictyol and thymonin, acting as GPR35 agonists. Both eriodictyol and thymonin showed a potent and specific agonist activity toward GPR35 with half maximal effective concentration values of 5.48 and 8.41 µm, respectively. These findings indicate that these phytochemicals may have beneficial health effects upon GPR35 activation.</jats:p>

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