Bacterial Lipopolysaccharide Induces PD-L1 Expression and an Invasive Phenotype of Oral Squamous Cell Carcinoma Cells

DOI Web Site 参考文献53件 オープンアクセス
  • Yuji Omori
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Hyogo Medical University, Mukogawa-cho1-1, Nishinomiya 663-8501, Japan
  • Kazuma Noguchi
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Hyogo Medical University, Mukogawa-cho1-1, Nishinomiya 663-8501, Japan
  • Mizuha Kitamura
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Hyogo Medical University, Mukogawa-cho1-1, Nishinomiya 663-8501, Japan
  • Yuna Makihara
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Hyogo Medical University, Mukogawa-cho1-1, Nishinomiya 663-8501, Japan
  • Takayuki Omae
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Hyogo Medical University, Mukogawa-cho1-1, Nishinomiya 663-8501, Japan
  • Soutaro Hanawa
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Hyogo Medical University, Mukogawa-cho1-1, Nishinomiya 663-8501, Japan
  • Kyohei Yoshikawa
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Hyogo Medical University, Mukogawa-cho1-1, Nishinomiya 663-8501, Japan
  • Kazuki Takaoka
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Hyogo Medical University, Mukogawa-cho1-1, Nishinomiya 663-8501, Japan
  • Hiromitsu Kishimoto
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Hyogo Medical University, Mukogawa-cho1-1, Nishinomiya 663-8501, Japan

説明

<jats:p>Background: Expression of programmed death ligand-1 (PD-L1) is related to the prognosis of many solid malignancies, including oral squamous cell carcinoma (OSCC), but the mechanism of PD-L1 induction remains obscure. In this study, we examined the expression of PD-L1 and partial epithelial–mesenchymal transition (pEMT) induced by bacterial lipopolysaccharide (LPS) in OSCC. Methods: The expression of Toll-like receptor 4 (TLR4) recognizing LPS in OSCC cell lines was analyzed. Moreover, the induction of PD-L1 expression by Porphyromonas gingivalis (P.g) or Escherichia coli (E. coli) LPS and EMT was analyzed by western blotting and RT-PCR. Morphology, proliferation, migration, and invasion capacities were examined upon addition of LPS. PD-L1 within EXOs was examined. Results: PD-L1 expression and pEMT induced by LPS of P.g or E. coli in TLR4-expressing OSCC cell lines were observed. Addition of LPS did not change migration, proliferation, or cell morphology, but increased invasive ability. Moreover, higher expression of PD-L1 was observed in OSCC EXOs with LPS. Conclusion: Oral bacterial LPS is involved in enhanced invasive potential in OSCC cells, causing PD-L1 expression and induction of pEMT. The enhancement of PD-L1 expression after addition of LPS may be mediated by EXOs.</jats:p>

収録刊行物

  • Cancers

    Cancers 16 (2), 343-, 2024-01-13

    MDPI AG

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