4,420,000 Yen
(Direct Cost: 3,400,000 Yen Indirect Cost: 1,020,000 Yen)
Research Abstract
There are multiple types of membrane proteins on the cell membrane, and regulation of these proteins leads to the improvement and control of cellular functions. In this study, we aimed to develop an "integrated bioconjugation" technology that enables the integration of multiple types of biomolecules on a single structure with controlled localization of each molecule. To this end, we focused on the development of scaffold to hold biomolecules that act on membrane proteins and the functionalization method of the biomolecules. We could successfully form self-assembly structures with domain structures by designing peptide amphiphiles (PAs) with immiscible substituents in the hydrophobic part. We also showed that biomolecules can be post-modified on PA assemblies by enzymatic reactions and avidin-biotin interactions.
