Molecular mechanisms of the fetal left-right axis malformations induced by maternal hyperglycemia.
-
- Kitajima Keiko
- Principal Investigator
- 九州大学
About this project
- Japan Grant Number
- JP18K07880
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding organization
- Japan Society for the Promotion of Science
- Project/Area Number
- 18K07880
- Research Category
- Grant-in-Aid for Scientific Research (C)
- Allocation Type
-
- Multi-year Fund
- Review Section / Research Field
-
- Basic Section 52050:Embryonic medicine and pediatrics-related
- Research Institution
-
- Kyushu University
- Project Period (FY)
- 2018-04-01 〜 2023-03-31
- Project Status
- Completed
- Budget Amount*help
- 4,420,000 Yen (Direct Cost: 3,400,000 Yen Indirect Cost: 1,020,000 Yen)
Research Abstract
The expression of Nodal and Cerl2 was decreased in the node of 8.0-day embryo exposed to high glucose concentration, and it was due to the reduction of active form of NOTCH (NICD) in the node. We focused on Wnt3a which is required for the expression of NOTCH around the node in 8.0-day embryo, and identified two enhancers, PSE1 and PSE2, for primitive streak specific expression of Wnt3a. We first introduced point mutation in Wnt3a PSE1 by CRISPR/Cas9 system, but there was no significant decrease in expression of Wnt3a in the pimitive streak, and the obtained homozygote pups were viable.