- 【Updated on May 12, 2025】 Integration of CiNii Dissertations and CiNii Books into CiNii Research
- Trial version of CiNii Research Knowledge Graph Search feature is available on CiNii Labs
- 【Updated on June 30, 2025】Suspension and deletion of data provided by Nikkei BP
- Regarding the recording of “Research Data” and “Evidence Data”
Generation of super thraustochytrids specialized for lipid production
-
- OKINO NOZOMU
- Principal Investigator
- 九州大学
-
- 石橋 洋平
- Co-Investigator
- 九州大学
About This Project
- Japan Grant Number
- JP18K19183 (JGN)
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding Organization
- Japan Society for the Promotion of Science
Kakenhi Information
- Project/Area Number
- 18K19183
- Research Category
- Grant-in-Aid for Challenging Research (Exploratory)
- Allocation Type
-
- Multi-year Fund
- Review Section / Research Field
-
- Medium-sized Section 38:Agricultural chemistry and related fields
- Research Institution
-
- Kyushu University
- Project Period (FY)
- 2018-06-29 〜 2022-03-31
- Project Status
- Completed
- Budget Amount*help
- 6,240,000 Yen (Direct Cost: 4,800,000 Yen Indirect Cost: 1,440,000 Yen)
Research Abstract
In this study, we aimed to isolate a thraustochytrid with enhanced lipid production and develop a genome editing workflow for its synthesis. Through breeding by focusing on the fact that the specific gravity of cells decreases with lipid accumulation, we succeeded in isolating a strain with improved saturated fatty acid production. Gene expression analysis of this strain revealed an enhanced expression of the fatty acid synthase gene. Next, we screened a mutant library where a drug resistance gene was inserted into the genome. This revealed that mitochondrial β-oxidation is vital for lipid metabolism in thraustochytrids. We also established an optimized protocol for thraustochytrid genome editing using the CRISPR/Cas9 technology.
Details 詳細情報について
-
- CRID
- 1040000782006047360
-
- Text Lang
- ja
-
- Data Source
-
- KAKEN