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Rapid detection of mutation in HIV-1 reverse transcriptase gene by temperature gradient gel electrophoresis
About This Project
- Japan Grant Number
- JP18KK0285 (JGN)
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding Organization
- Japan Society for the Promotion of Science
Kakenhi Information
- Project/Area Number
- 18KK0285
- Research Category
- Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
- Allocation Type
-
- Multi-year Fund
- Review Section / Research Field
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- Medium-sized Section 62:Applied informatics and related fields
- Research Institution
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- Kyoto University
- Project Period (FY)
- 2019-02-07 〜 2023-03-31
- Project Status
- Completed
- Budget Amount*help
- 17,680,000 Yen (Direct Cost: 13,600,000 Yen Indirect Cost: 4,080,000 Yen)
Research Abstract
The ultimate goal of this research is to establish a novel method to isothermally amplify a target DNA from clinical specimen. Following DNA amplification, micro temperature gradient gel electrophoresis (TGGE) and analysis of DNA pattern were performed using image processing technology to identify mutations. For this purpose, we have carried out the following: 1) Examination of the effects of length and mutational position on the TGGE pattern; 2) Development of a micro TGGE apparatus capable of accommodating 2-5 gels in one electrophoresis cycle; 3) Development of a software to detect inflection points in a TGGE pattern; 4) Alteration of enzymes utilized in an isothermal DNA amplification method, including recombinase polymerase amplification; 5) Construction of networks to obtain clinical specimen in India.
Keywords
Details 詳細情報について
-
- CRID
- 1040000782006617984
-
- Text Lang
- ja
-
- Data Source
-
- KAKEN
- IRDB