Development of methods for genetic analysis of malignant lymphoma using cell free DNA from peripheral blood and FFPE DNA

KAKEN

About This Project

Japan Grant Number
JP26893107 (JGN)
Funding Program
Grants-in-Aid for Scientific Research
Funding Organization
Japan Society for the Promotion of Science

Kakenhi Information

Project/Area Number
26893107
Research Category
Grant-in-Aid for Research Activity Start-up
Allocation Type
  • Single-year Grants
Review Section / Research Field
  • Biological Sciences > Medicine, Dentistry, and Pharmacy > Clinical internal medicine > Hematology
Research Institution
  • Nagoya University
Project Period (FY)
2014-08-29 〜 2016-03-31
Project Status
Completed
Budget Amount*help
2,990,000 Yen (Direct Cost: 2,300,000 Yen Indirect Cost: 690,000 Yen)

Research Abstract

Analysis were performed using cell free DNA (cfDNA) from peripheral blood and DNA from formalin fixed paraffin embedded tissue (FFPE DNA) of patients with diffuse large B cell lymphoma (DLBCL). Concentration of cfDNA varied among patients (0.23 to 547 ng in 1ml plasma), and changed during chemotherapy. cfDNA was detected as ladder pattern. Amplification of 300 to 600 base pairs of PCR products was successfully performed using cfDNA. Genomic mutations which were detected in biopsy specimen can be detected in cfDNA. It is suggested that cfDNA can be alternative source for genetic analysis which can be obtained less invasively and frequently in order to detect genetic alteration time-dependently. FFPE DNA was detected as smear pattern and sometimes it was difficult to amplify DNA with PCR for some samples. However it enables us to analyze genetic abnormalities retrospectively for many cases whose fresh biopsy samples could not be acquired enough.

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