Induction and detection of retroposition of SINEs and LINEs in animal genome

KAKEN
  • OKADA Norihiro
    Principal Investigator
    Tokyo Institute of Technology, Graduate School of Bioscience & Biotechnology, Professor
  • OHSHIMA Kazuhiko
    Co-Investigator
    Tokyo Institute of Technology, Graduate School of Bioscience & Biotechnology, Lecturer

About This Project

Japan Grant Number
JP13680759 (JGN)
Funding Program
Grants-in-Aid for Scientific Research
Funding Organization
Japan Society for the Promotion of Science

Kakenhi Information

Project/Area Number
13680759
Research Category
Grant-in-Aid for Scientific Research (C)
Allocation Type
  • Single-year Grants
Review Section / Research Field
  • Multidisciplinary Fields > 基礎生物科学 > Molecular biology
Research Institution
  • Tokyo Institute of Technology
Project Period (FY)
2001 〜 2002
Project Status
Completed
Budget Amount*help
3,600,000 Yen (Direct Cost: 3,600,000 Yen)

Research Abstract

We characterized members of the LINE (UnaL2) and SINE (UnaSINE1) families from the eel genome and found that these LINE/SINE partners share similar 3' tails. A retrotransposition assay in HeLa cells demonstrated that the 3' conserved tail of UnaL2 is necessary for its retrotransposition. This 3' tail is recognized in trans by the UnaL2 reverse transcriptase at a surprisingly high rate, and that of UnaSINE1 can also be recognized, thus providing experimental evidence that a SINE can be mobilized by the retrotransposition machinery of a partner LINE. We also demonstrated that short repeats at the 3' end of UnaL2 are required for retrotransposition suggesting that UnaL2 retrotransposes in a manner reminiscent of the reverse transcriptase activity of telomerases.

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