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Induction and detection of retroposition of SINEs and LINEs in animal genome
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- OKADA Norihiro
- Principal Investigator
- Tokyo Institute of Technology, Graduate School of Bioscience & Biotechnology, Professor
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- OHSHIMA Kazuhiko
- Co-Investigator
- Tokyo Institute of Technology, Graduate School of Bioscience & Biotechnology, Lecturer
About This Project
- Japan Grant Number
- JP13680759 (JGN)
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding Organization
- Japan Society for the Promotion of Science
Kakenhi Information
- Project/Area Number
- 13680759
- Research Category
- Grant-in-Aid for Scientific Research (C)
- Allocation Type
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- Single-year Grants
- Review Section / Research Field
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- Multidisciplinary Fields > 基礎生物科学 > Molecular biology
- Research Institution
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- Tokyo Institute of Technology
- Project Period (FY)
- 2001 〜 2002
- Project Status
- Completed
- Budget Amount*help
- 3,600,000 Yen (Direct Cost: 3,600,000 Yen)
Research Abstract
We characterized members of the LINE (UnaL2) and SINE (UnaSINE1) families from the eel genome and found that these LINE/SINE partners share similar 3' tails. A retrotransposition assay in HeLa cells demonstrated that the 3' conserved tail of UnaL2 is necessary for its retrotransposition. This 3' tail is recognized in trans by the UnaL2 reverse transcriptase at a surprisingly high rate, and that of UnaSINE1 can also be recognized, thus providing experimental evidence that a SINE can be mobilized by the retrotransposition machinery of a partner LINE. We also demonstrated that short repeats at the 3' end of UnaL2 are required for retrotransposition suggesting that UnaL2 retrotransposes in a manner reminiscent of the reverse transcriptase activity of telomerases.
Keywords
Details 詳細情報について
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- CRID
- 1040282256746325632
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- Text Lang
- ja
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- Data Source
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- KAKEN