4,810,000 Yen
(Direct Cost: 3,700,000 Yen Indirect Cost: 1,110,000 Yen)
Research Abstract
With regard to epigenetic mutation that are considered to be involved in diseases such as carcinogenesis, there is no validated detection system for epigenetic mutation. In this study, we attempted to construct a bioassay system that can easily detect epigenetic mutation with a microorganism. Budding yeast is an eukaryotic single-celled microorganism. It has been found that the yeasts transformed with cDNAs of human DNA methyltransferase genes apparently show a flocculation phenotype. Therefore, we tried to construct an epigenetic mutation detection system based on a reporter assay using the FLO1 flocculation gene. In this study, our findings suggest that yeast flocculation is a useful marker for the detection of epigenetic mutation.
