Trials to strengthen bacterial cell membrane by the production of archaeal membrane lipids
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- Hemmi Hisashi
- Principal Investigator
- 名古屋大学
-
- MOTOYAMA Kento
- Research Collaborator
- 名古屋大学
-
- KOKUBO Takuya
- Research Collaborator
- 名古屋大学
-
- YOSHIDA Ryo
- Research Collaborator
- 名古屋大学
About This Project
- Japan Grant Number
- JP16K14882 (JGN)
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding Organization
- Japan Society for the Promotion of Science
Kakenhi Information
- Project/Area Number
- 16K14882
- Research Category
- Grant-in-Aid for Challenging Exploratory Research
- Allocation Type
-
- Multi-year Fund
- Review Section / Research Field
-
- Biological Sciences > Agriculture > Agricultural chemistry > Applied microbiology
- Research Institution
-
- Nagoya University
- Project Period (FY)
- 2016-04-01 〜 2018-03-31
- Project Status
- Completed
- Budget Amount*help
- 3,770,000 Yen (Direct Cost: 2,900,000 Yen Indirect Cost: 870,000 Yen)
Research Abstract
The structures of membrane lipids from archaea are different from those from other organisms, bacteria and eukaryotes. The difference causes the low permeability of membrane composed of archaeal membrane lipids to small molecules such as ions, which is considered to enable archaea to grow under extreme conditions. Thus, we have forced the bacterium Escherichia coli to produce archaeal membrane lipids in order to strengthen its cell membrane. In this study, we introduced a biosynthetic pathway of the precursors of archaeal membrane lipids in E. coli to increase their production levels. And, we elucidated the biosynthetic pathway of membrane lipids specific to a hyperthermophilic archaeon, which are expected to have greater effects on the strengthening of membrane, and constructed the production system of the lipids in E. coli.
