Development of methodology using environmental DNA to monitor insect pathogens of forest defoliating insects

KAKEN

About This Project

Japan Grant Number
JP17H03826 (JGN)
Funding Program
Grants-in-Aid for Scientific Research
Funding Organization
Japan Society for the Promotion of Science

Kakenhi Information

Project/Area Number
17H03826
Research Category
Grant-in-Aid for Scientific Research (B)
Allocation Type
  • Single-year Grants
Review Section / Research Field
  • Biological Sciences > Agriculture > Forest and forest products science > Forest science
Research Institution
  • The University of Tokyo
Project Period (FY)
2017-04-01 〜 2021-03-31
Project Status
Completed
Budget Amount*help
15,860,000 Yen (Direct Cost: 12,200,000 Yen Indirect Cost: 3,660,000 Yen)

Research Abstract

Spatial variation in fungal communities in forest soils is high. Entomopathogenic fungi include important natural enemies of forest insects, but their information is limited except during outbreaks, partly because many of them live in soil. The objective of this study was to develop a method for monitoring entomopathogenic fungi in soil using environmental DNA methods. Three different methods were used to extract DNA from 0.4 g (according to a kit recipe) and 15 g (intention to collect fungal DNA more widely) of soil volume. Amplicon analysis was performed using next-generation sequencers. Contrary to expectations, the smaller the amount of soil used for extraction, the greater number of fungal OTU were detected. The results of this study indicate that the best strategy is to sample a large number of small soil samples.

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