Mechanisms of shell spiral growth: a primer for Paleo-Evo-Devo
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- Endo Kazuyoshi
- Principal Investigator
- 東京大学
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- 野下 浩司
- Co-Investigator
- 九州大学
About this project
- Japan Grant Number
- JP18H01323
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding organization
- Japan Society for the Promotion of Science
- Project/Area Number
- 18H01323
- Research Category
- Grant-in-Aid for Scientific Research (B)
- Allocation Type
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- Single-year Grants
- Review Section / Research Field
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- Basic Section 17050:Biogeosciences-related
- Research Institution
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- The University of Tokyo
- Project Period (FY)
- 2018-04-01 〜 2023-03-31
- Project Status
- Completed
- Budget Amount*help
- 17,160,000 Yen (Direct Cost: 13,200,000 Yen Indirect Cost: 3,960,000 Yen)
Research Abstract
In this project, we developed basic know-hows to perform genome editing using CRISPR/Cas9 in the limpet Nipponacmea fuscoviridis, and we also carried out experiments to unveil the genetic mechanisms underlying the shell spiral growth in molluscs. As a result, we successfully knocked out the lophotrochin gene which is known to be expressed in an early larval stage in the limpet. We also identified several shell matrix protein genes which are unevenly expressed between the right and left sides of the mantle, and could be related to the lateral asymmetry of the shell growth in the pond snail Lymnaea stagnalis. We further discovered that the signal transduction factor Wnt is responsible for the twisted growth of the shell during the spiral shell growth in L. stagnalis.