Trans-omic study for diversity and evolution of venomic proteins of Protobothrops flavoviridis.
-
- Shibata Hiroki
- Principal Investigator
- 九州大学
-
- 小川 智久
- Co-Investigator
- 東北大学
About this project
- Japan Grant Number
- JP18H02498
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding organization
- Japan Society for the Promotion of Science
- Project/Area Number
- 18H02498
- Research Category
- Grant-in-Aid for Scientific Research (B)
- Allocation Type
-
- Single-year Grants
- Review Section / Research Field
-
- Basic Section 45030:Biodiversity and systematics-related
- Research Institution
-
- Kyushu University
- Project Period (FY)
- 2018-04-01 〜 2022-03-31
- Project Status
- Completed
- Budget Amount*help
- 17,290,000 Yen (Direct Cost: 13,300,000 Yen Indirect Cost: 3,990,000 Yen)
Research Abstract
The whole genome assembly of habu individuals from four populations, Amami (Protobothrops flavoviridis), Okinawa (P. flavoviridis), Kodakara (P. tokarensis) and Iriomote (P. elegans) revealed different structures in gene clusters of venom protein gene families such as metalloproteinase and C-type lectin genes (supported by JSPS KAKENHI Grant Number 22H04925 (PAGS)). We also obtained mtDNA sequences from 150 individuals from 14 populations and evaluated the genetic divergence and migrations among the populations. 2D analyses of venoms from 47 individuals from 9 populations showed different expression pattern between populations as well as within populations, while RNAseq of venom glands showed no clear difference in expression levels of venom protein genes between populations. These observations suggest that the difference in protein expression levels in venoms can be attributed to genomic, translational and post-translational levels rather than transcriptional levels.
Details 詳細情報について
-
- CRID
- 1040282256969958912
-
- Text Lang
- ja
-
- Data Source
-
- KAKEN