LLB Phage: Novel approach to eliminate food poisoning bacteria
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- Masuda Yoshimitsu
- Principal Investigator
- 九州大学
About this project
- Japan Grant Number
- JP18K14378
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding organization
- Japan Society for the Promotion of Science
- Project/Area Number
- 18K14378
- Research Category
- Grant-in-Aid for Early-Career Scientists
- Allocation Type
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- Multi-year Fund
- Review Section / Research Field
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- Basic Section 38020:Applied microbiology-related
- Research Institution
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- Kyushu University
- Project Period (FY)
- 2018-04-01 〜 2022-03-31
- Project Status
- Completed
- Budget Amount*help
- 4,030,000 Yen (Direct Cost: 3,100,000 Yen Indirect Cost: 930,000 Yen)
Research Abstract
This study was designed to construct a novel antimicrobial agent, LLB-producing phage (LLB-phage) against pathogenic bacteria. As a result of this study, the first LLB-phage lnqQ-T7, LLB-ECP52s targeting enterohemorrhagic Escherichia coli, and lnqQ-vPSARa targeting multi-drug resistant Staphylococcus aureus were constructed. lnqQ-T7 was constructed by trx-dependent homologous recombination system and LLB-ECP52s were constructed by genome editing system with CRISPR-Cas9. They could control the growth of not only E. coli host strain but neighboring gram-positive bacteria by enhanced lytic activity and LLB production. lnqQ-T7 could also inhibit an emergence of phage resistant population. MRSA targeting LLB phage lnqQ-vPSARa was constructed by editing system with CRISPR-Cas10, in which we could transform temperate phage PSARa into virulent phage by deleting 4 putative lysogenic genes. This study demonstrated the great possibility that we can design various types of LLB-phages on demand.
Keywords
Details 詳細情報について
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- CRID
- 1040282256980557312
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- Text Lang
- ja
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- Data Source
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- KAKEN
- IRDB
