Exploration of the mechanism(s) of cartilage degeneration in osteoarthritis through the analysis of exosomes in the synovial fluid.
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- Tsuno Hirotaka
- Principal Investigator
- 独立行政法人国立病院機構(相模原病院臨床研究センター)
About this project
- Japan Grant Number
- JP18K16638
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding organization
- Japan Society for the Promotion of Science
- Project/Area Number
- 18K16638
- Research Category
- Grant-in-Aid for Early-Career Scientists
- Allocation Type
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- Multi-year Fund
- Review Section / Research Field
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- Basic Section 56020:Orthopedics-related
- Research Institution
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- Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital
- Project Period (FY)
- 2018-04-01 〜 2021-03-31
- Project Status
- Completed
- Budget Amount*help
- 4,160,000 Yen (Direct Cost: 3,200,000 Yen Indirect Cost: 960,000 Yen)
Research Abstract
Although pain is the most critical symptom of osteoarthritis (OA). Recent epidemiological studies have shown that the synovial pathology is closely related to pain in OA knees. However, the mechanisms underlying synovial pathology in OA has not yet been clarified, and the same is true for the reasons why pain is associated with the synovial pathology. In this study, we focused on miRNA involved in the pain in OA knees. To that end, synovial fluid (SF) samples were collected from OA knees twice at the peak of pain and after the remission of pain, and exosomes were obtained from these SF samples. miRNA was extracted from the exosomes and the miRNAs related to pain were analyzed by qPCR analyses. The results were compared between the SF samples from respective knees, and miRNA was determined the concentrations of which differed significantly between the two points of time. In results, a total of 5 miRNAs were found which could be related to pain in OA knee joints.