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live-cell hybrid imaging of morphological dynamics of the plasma membrane
About This Project
- Japan Grant Number
- JP18KK0196 (JGN)
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding Organization
- Japan Society for the Promotion of Science
Kakenhi Information
- Project/Area Number
- 18KK0196
- Research Category
- Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
- Allocation Type
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- Multi-year Fund
- Review Section / Research Field
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- Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
- Research Institution
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- Kyoto University
- Project Period (FY)
- 2018-10-09 〜 2022-03-31
- Project Status
- Completed
- Budget Amount*help
- 17,940,000 Yen (Direct Cost: 13,800,000 Yen Indirect Cost: 4,140,000 Yen)
Research Abstract
We established a hybrid live-cell imaging system by combining high-speed atomic force microscopy (HS-AFM) and fluorescence microscopy and revealed the detailed morphological changes in the plasma membrane and protein localizations during the clathrin-mediated endocytosis. CIP4, which has membrane-deforming activity, is recruited to the plasma membrane via small G protein Cdc42, and promotes the assembly of actin, which induces a small membrane bulge near the clathrin pit. We also established a hybrid microscope system by combining HS-AFM and highly inclined and laminated optical sheet (HILO) illumination to overcome the limitation of light diffraction, and successfully visualized a single molecule in the plasma membrane by super-resolution radial fluctuation.
Details 詳細情報について
-
- CRID
- 1040282256983278208
-
- Text Lang
- ja
-
- Data Source
-
- KAKEN