Synthesis of a Versatile Probe for Analysis of Cytoplasmic Peptide-N-Glycanase

KAKEN

About This Project

Japan Grant Number
JP21580410 (JGN)
Funding Program
Grants-in-Aid for Scientific Research
Funding Organization
Japan Society for the Promotion of Science

Kakenhi Information

Project/Area Number
21580410
Research Category
Grant-in-Aid for Scientific Research (C)
Allocation Type
  • Single-year Grants
Review Section / Research Field
  • Biological Sciences > Agriculture > Boundary agriculture > Applied molecular and cellular biology
Research Institution
  • Gunma University
Project Period (FY)
2009 〜 2011
Project Status
Completed
Budget Amount*help
4,940,000 Yen (Direct Cost: 3,800,000 Yen Indirect Cost: 1,140,000 Yen)

Research Abstract

Functions of Peptide-N-Glycanase (PNGase) in endoplasmic reticulum-associated degradation (ERAD) are attracting recent attention. In order to analyze in this process, we developed a potent inhibitor having clickable tag that can allow for a variety of functional groups to be introduced such as detection and purification tags easily using a Cu (I)-catalyzed [3,2] cycloaddition reaction. Our probe inhibited PNGase selectively by micro M order and can easily lead to a variety of compounds. These compounds should help in the understanding of the biological functions of cytoplasmic PNGase.

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