Single-Molecule Sequencing Methods via Tunneling Current
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- Taniguchi Masateru
- Principal Investigator
- 大阪大学
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- TSUTSUI Makusu
- Research Collaborator
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- OHSHIRO Takahito
- Research Collaborator
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- YOKOTA Kazumichi
- Research Collaborator
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- KOMOTO Yuki
- Research Collaborator
About This Project
- Japan Grant Number
- JP26220603 (JGN)
- Funding Program
- Grants-in-Aid for Scientific Research
- Funding Organization
- Japan Society for the Promotion of Science
Kakenhi Information
- Project/Area Number
- 26220603
- Research Category
- Grant-in-Aid for Scientific Research (S)
- Allocation Type
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- Single-year Grants
- Review Section / Research Field
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- Science and Engineering > Interdisciplinary science and engineering > Nano/Micro science > Nanomaterials chemistry
- Research Institution
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- Osaka University
- Project Period (FY)
- 2014-05-30 〜 2019-03-31
- Project Status
- Completed
- Budget Amount*help
- 177,710,000 Yen (Direct Cost: 136,700,000 Yen Indirect Cost: 41,010,000 Yen)
Research Abstract
In this study, we successfully concatenated the base molecules in DNA and RNA and the partial amino acid sequence of peptide and sequenced the entire genome of a virus by measuring the tunneling current flowing through a single molecule. We successfully observed the direct incorporation of our target anticancer drug into DNA and identified the individual molecules associated with the chemically modified base molecule and the amino acid, both of which are vital disease markers. A quantitative method was developed to analyze the base sequences and abundance ratios for the two types of DNA and RNA contained in the target solution. We determined the amino acid sequences and the abundance ratios for the two types of peptides under investigation.
Details 詳細情報について
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- CRID
- 1040282257257108992
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- Text Lang
- ja
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- Data Source
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- KAKEN
