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説明
Green fluorescent protein (GFP)-tagged tubulin has been widely employed to examine tubulin dynamics in living cells. In the present study, we biochemically re-evaluate the property of GFP-tubulin expressed in cells. When expressed in mammalian cells, antibodies against GFP or the amino-terminal region of tubulin detected GFP-tubulin in western blot analyses. By contrast, antibodies against the carboxyl terminal region of tubulin failed to recognize exogenous GFP-tubulin, but not endogenous tubulin. These results indicate that GFP-tubulin is partially truncated at the carboxyl terminal region and suggest that data of experiments using GFP-tubulin should be carefully analyzed.
収録刊行物
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- 理工学総合研究所研究報告
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理工学総合研究所研究報告 (21), 19-23, 2009-02-01
近畿大学理工学総合研究所
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詳細情報 詳細情報について
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- CRID
- 1050001202539613056
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- NII論文ID
- 120005737150
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- NII書誌ID
- AN10074306
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- ISSN
- 09162054
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- NDL書誌ID
- 10215466
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- 本文言語コード
- en
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- 資料種別
- departmental bulletin paper
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- データソース種別
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- IRDB
- NDL
- CiNii Articles