INCREASING LEVELS OF AMPHIREGULIN IN FOLLICULAR FLUIDS ARE ASSOCIATED WITH HUMAN OOCYTE MATURATION
この論文をさがす
抄録
Luteinizing hormone stimulates ovarian somatic cells to induce hormone release for oocyte maturation. Accumulating evidences in rodents indicate importance of epidermal growth factor (EGF)-like growth factors [amphiregulin (AREG), epiregulin (EREG) and betacellulin (BTC)] from the somatic cells in oocyte maturation. Here, we investigated an involvement of ovarian EGF-like growth factors in human oocyte maturation. Using isolated human ovarian cells, we found the expression of AREG, EREG and BTC mRNAs in granulosa and cumulus cells by RT-PCR, and AREG protein in cumulus cells, suggesting their production in ovarian somatic cells. In human follicular fluids aspirated from individual follicles undergoing in vitro fertilization-embryo transfer and granulosa cells obtained by centrifugation of the fluid, the association between AREG, EREG, and BTC levels and oocyte maturation was examined by using enzyme-linked immunosorbent assay or quantitative real-time RT-PCR................................. ...............................AREG protein level was significantly higher in the follicular fluids that yielded mature oocytes. However, EREG mRNA level in granulosa cells was insignificant between mature and immature oocytes, and BTC protein was absent from all follicular fluids examined. Our findings suggest an important role for AREG, but not EREG and BTC, in optimal maturation of human oocytes.
収録刊行物
-
- 秋田医学
-
秋田医学 37 (1), 43-50, 2010-01-01
秋田医学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1050001202562082176
-
- NII論文ID
- 110008436656
-
- NII書誌ID
- AN00009294
-
- ISSN
- 03866106
-
- HANDLE
- 10295/1800
-
- NDL書誌ID
- 10771606
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- IRDB
- NDL
- CiNii Articles