LIVE IMAGING BY TIME-LAPSE MICROSCOPY CAN MORE CLEARLY EVALUATE THE ANTI-APOPTOTIC STATE OF PRIMARY HEPATOCYTES ISOLATED FROM THE DRS KNOCKOUT MOUSE
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説明
To investigate the anti-apoptotic state of primary hepatocytes isolated from the drs knockout(KO)mice, Fas-mediated apoptosis was observed by flow cytometry and time-lapse microscopy. Treatment with anti-Fas antibody plus actinomycin D or cycloheximide induced over 50% apoptosis for primary hepatocytes based on annexin V staining. At 24 hr later under these apoptotic stimuli, the sub G1 fraction of hepatocytes isolated from the drs KO mice was significantly decreased in comparison to wild C57BL/ 6 mice. In contrast, sequential time-lapse microscopic analysis revealed clearer and larger differences in the apoptotic responses of primary hepatocytes from the drs KO and wild-type mice from 4 hr to 24 hr after apoptotic induction. The time-lapse microscopic analysis clearly indicated the hepatocytes isolated from the drs KO mouse to be more resistant to the apoptotic stimuli than other methods. The time-lapse microscopy is very useful for the sequential observation of viable primary hepatocytes.
収録刊行物
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- 秋田医学
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秋田医学 35 (3/4), 231-237, 2009-03-01
秋田医学会
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詳細情報 詳細情報について
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- CRID
- 1050001202563313152
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- NII論文ID
- 110007092989
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- NII書誌ID
- AN00009294
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- ISSN
- 03866106
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- HANDLE
- 10295/1624
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- NDL書誌ID
- 10217514
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- NDL
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