Reciprocal complementation of bovine parainfluenza virus type 3 lacking either the membrane or fusion gene
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Abstract
Two defective bovine parainfluenza virus type 3 (BPIV3) strains were generated, one lacking the membrane (M) protein gene and expressing EGFP (ΔM-EGFP) and the other lacking the fusion (F) protein gene and expressing mStrawberry (ΔF-mSB), by supplying deficient proteins in trans. When Madin-Darby bovine kidney (MDBK) cells were co-infected with ΔM-EGFP and ΔF-mSB at a multiplicity of infection (MOI) of 0.1, complemented viruses were easily obtained. Complemented viruses grew as efficiently as wild-type BPIV3 and could be passaged in MDBK cell cultures even at an MOI of 0.01, possibly due to multiploid virus particles containing genomes of both ΔM-EGFP and ΔF-mSB. This reciprocal complementation method using two defective viruses would be useful to express large or multiple proteins in cell cultures using paramyxovirus vectors.
Journal
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- Journal of virological methods
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Journal of virological methods 249 25-30, 2017-11
Elsevier
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Details
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- CRID
- 1050001202640975488
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- NII Article ID
- 120007128817
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- NII Book ID
- AA00259584
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- ISSN
- 01660934
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- HANDLE
- 2241/00149150
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- CiNii Articles