Bibliographic Information
- Other Title
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- 海洋における細菌計数法の比較 : 4'6-diamidino-2-phenylindole (DAPI)計数法versus SYBR Green I計数法
- カイヨウ ニ オケル サイキン ケイスウホウ ノ ヒカク 4 6 diamidino 2 phenylindole DAPI ケイスウホウ versus SYBR GREEN 1 ケイスウホウ
- Comparison of two epifluorescence counting methods of bacteria in the Ocean : 4'6-diamidino-2-phenylindole (DAPI) versus SYBR Green I method
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Abstract
We investigated the availability of a recentdeveloped nucleic acid stain, SYBR Green I, bacterial counting method. Comparison of bacterial counts made with SYBR Green I and DAPI method was conducted using coastal seawater samples. Bacterial counts with both methods were significantly correlated (r=0.60, n=23). However, the ratio of bacterial counts with SYBR Green I and DAPI method was 2.0±0.5, indicating that the count with SYBR green I method was about twice higher than with DAPI method. Bacteria were intensively stained with SYBR Green I and easier to observe under epifluorescence microscopy than with DAPI method. Although it is difficult to identify the reason for difference of the bacterial counts, our results imply a possible existence of bacteria which cannot be detected with the DAPI method used previously.
Journal
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- Actinia : bulletin of the Manazuru Marine Laboratory for Science Education, Faculty of Education and Human Sciences, Yokohama National University
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Actinia : bulletin of the Manazuru Marine Laboratory for Science Education, Faculty of Education and Human Sciences, Yokohama National University 14 31-36, 2001-12-31
横浜国立大学教育人間科学部附属理科教育実習施設
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Details 詳細情報について
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- CRID
- 1050001202679508736
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- NII Article ID
- 110006151677
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- NII Book ID
- AA11639270
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- NDL BIB ID
- 6269053
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- Text Lang
- ja
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- Article Type
- departmental bulletin paper
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- Data Source
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- IRDB
- NDL
- CiNii Articles