スナネズミの一過性前脳虚血における海馬グリア細胞の変化 : 発達段階を中心として

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  • The glial reactions after transient ischemia in developing gerbil hippocampus
  • スナネズミ ノ イッカセイ ゼンノウ キョケツ ニ オケル カイバ グリア サイボウ ノ ヘンカ : ハッタツ ダンカイ ヲ チュウシン トシテ

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In animal models of transients ischemia, selective vulnerability and delayed neuronal death (DND)in the hippocampus have been extensively described. It is well known that the developing brains have toleranceto damage. In the present study, we investigated the critical stage of the damage of DND in the developinggerbils and the differences of glial reactions within a week after transient ischemia in two groupswith and without DND. The gerbils suffered from transient ischemia in various postnatal days were all perfusedat 10 weeks-old(adult). Histopathological evaluation of DND was performed by cresil violet staining.DND was not seen before 3 weeks-old. Glial reactions were examined immunohistochemically by the antibodiesagainst GFAP, Mac-1 and CNPase, for astrocyte, microglia and oligodendrocyte, respectively. Themost interesting reactive changes were observed in GFAP immunostaining. Computer image analysisshowed that area fraction of GFAP-positive structures in CA1 region was significantly increased in bothischemic cases with or without DND compared with each sham group. Especially in animals with DND in 3days after ischmia , the numerous astrocytes were observed even in the lateral half of st. pyramidalis, inwhich DND was just occurring, as estimated in cresil violet staining. The findings suggest that the astrocytesplay an important role in neuronal survival and death after iscemia in both cases with and withoutDND.

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