ＩＳＦＥＴバイオセンサシステムによるＬ－グルタミン定量の可能性の検討

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  • ISFET バイオセンサ システム ニヨル L グルタミン テイリョウ ノ カ

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A glutamine sensor was constructed by immobilizing L-glutaminase with glutaraldehyde on ISFET (ion selective field effect transistor) to investigate the possibility of determining L-glutamine. The prin- ciple of this sensor is as follows. The output voltage from ISFET, which depends on a very minute pH elevation due to the ammonium ion formation according to the enzymic reaction, is amplified with a differential amplifier of our own assembled. The amplified voltage was processed automatically with an on-line personal computer via GP-IB interface on a real-time basis. The calibration line for L-glutamine was linear from 1mM to 40mM and the relation coefficient was 0.967. The sensitivity of this system to L-glutamine was 0.5mM. The time needed for deter- mining one sample was within 13 min. The substrate specificity of this sensor was strict and L- glutamine was the sole compound responding to this sensor among analogues tested. D-glutamine was completely inactive to this sensor. It is suggested that L-glutanrine can be determined with this newly constructed ISFET-biosensor system with L-glutaminase.

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