Genetic manipulation of reptilian embryos: Toward an understanding of cortical development and evolution
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説明
The mammalian neocortex is a remarkable structure that is characterized by tangential surface expansion and six-layered lamination. However, how the mammalian neocortex emerged during evolution remains elusive. Because all modern reptiles have a homolog of the neocortex at the dorsal pallium, developmental analyses of the reptilian cortex are valuable to explore the origin of the neocortex. However, reptilian cortical development and the underlying molecular mechanisms remain unclear, mainly due to technical difficulties with sample collection and embryonic manipulation. Here, we introduce a method of embryonic manipulations for the Madagascar ground gecko and Chinese softshell turtle. We established in ovo electroporation and an ex ovo culture system to address neural stem cell dynamics, neuronal differentiation and migration. Applications of these techniques illuminate the developmental mechanisms underlying reptilian corticogenesis, which provides significant insight into the evolutionary steps of different types of cortex and the origin of the mammalian neocortex.
収録刊行物
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- Frontiers in Neuroscience
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Frontiers in Neuroscience 9 45-, 2015-01-01
Frontiers Media SA
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詳細情報 詳細情報について
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- CRID
- 1050001335832922112
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- NII論文ID
- 120005947014
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- ISSN
- 1662453X
- 16624548
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- HANDLE
- 2433/214303
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- PubMed
- 25759636
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE
