Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells
Description
We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation capacity and increased several pluripotent genes’ expression compared with the flat monolayer cells. We further demonstrated that cell-matrix adhesion mediates the interaction between cell morphology and expression of KLF4 and KLF5 through a serum response factor (SRF)-based regulatory double loop. Our results provide a mechanistic view on the coupling among adhesion, stem cell morphology, and pluripotency, shedding light on the critical role of cell-matrix adhesion in the induction and maintenance of hPSC.
Journal
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- Stem Cell Reports
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Stem Cell Reports 11 (1), 142-156, 2018-07-10
Elsevier BV
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Keywords
Details 詳細情報について
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- CRID
- 1050001335860224896
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- NII Article ID
- 120006488610
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- ISSN
- 22136711
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- HANDLE
- 2433/232589
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- Crossref
- CiNii Articles
- KAKEN