書誌事項
- タイトル別名
-
- ギョカイルイ ニ ソンザイ スル セイリ カッセイ リン シシツ タカ フホウワ シボウサン ガンユウ コリングリセロリン シシツ ノ カサンカ ト ノ カンレン
- Presence of Bioactive Phopsholipids in Fishes and Shellfishes : Peroxidation of PUFA containing cholineglycerophospholipids and PAF-like phospholipids
この論文をさがす
抄録
Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a potent bioactive lipid that is formed enzymatically in various mammalian cells and tissues. We found that high PAF activities in lipid extracts from fishes. Recently, Tanaka et al. reported that novel phospholipids with an sn-2-short-chain acyl groups, having PAF-like bioactivities, were produced by lipid peroxidation of CGP (cholineglycerophospholipid) with an sn-2-polyunsaturated fatty acyl (PUFA) group. Therefore, we wondered whether the bioactive phospholipids in lipid extract from fishes were formed by lipid peroxidation. We extracted and prepared lipids with and without an anti-oxidant BHT from the tissues of various kinds of fishes and shellfishes. The lipid extract containing PAF-like compound that was purified by thin-layer chromatography induced the aggregation of washed rabbit platelets, and the activities were inhibited by PAF receptor antagonist, WEB-2086. Fishes which had high PAF-like activities were abundant in CGP with PUFA. In the case of lipid preparations without BHT, increase in PAF-like activities and TBARS (thiobarbituric acid-reactive substances) values, and decrease in PUFA content in CGP were observed. Furthermore, we peroxidized CGPs derived from various fishes and shellfishes, and PAF-like biological activities were measured by platelet aggregation. Investigations of the correlation between the PAF-like activities produced by peroxidation of PUFA containing CGP and the contents of PUFA and alkyl ether-linked subclass in parent CGPs, revealed that higher activities were produced by peroxidation of alky-PUFA CGP species, that were rich in alkyl ether-linked subclass and/or rich in docosahexaenoate (DHA). These results suggest that the lipids having PAF-like biological activities in fishes and shellfishes were produced by peroxidation of PUFA containing CGP.
identifier:http://repo.kyoto-wu.ac.jp/dspace/handle/11173/1376
収録刊行物
-
- 京都女子大学食物学会誌
-
京都女子大学食物学会誌 053 31-39, 1998-12-10
京都女子大学食物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1050001337577391744
-
- NII論文ID
- 110000056243
-
- NII書誌ID
- AN00060473
-
- ISSN
- 02893827
-
- NDL書誌ID
- 4701254
-
- 本文言語コード
- ja
-
- 資料種別
- departmental bulletin paper
-
- データソース種別
-
- IRDB
- NDL
- CiNii Articles