Effect of substituting tryptophan for arginine on the cellular uptake of oligo-arginine peptides
Bibliographic Information
- Other Title
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- オリゴアルギニンペプチドの細胞内移行におけるTrp 残基導入の影響
- オリゴアルギニンペプチド ノ サイボウ ナイ イコウ ニ オケル Trpザンキ ドウニュウ ノ エイキョウ
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Abstract
Oligo-arginine peptides are short cationic peptides capable of traversing the plasma membranes of cells. In order to explore the effective substitutes Trp for Arg on the cellular uptake of oligo-arginine peptides, we designed and synthesized a series of Trp-containing analogues. All synthetic peptides did not show cytotoxicity on Jurkat cells at final concentration of 10μM.[Trp5]- and[Trp6]analogues exhibited moderate cellular uptake(about 75% of octaarginine, R8C), and[Trp6,8]analogue exhibited similar or even slightly increased cellular uptake as compared to R8C. The other synthetic peptides were showed poor cellular uptake(less than 50%). A clear relationship was not observed between hydrophobicity and cellular uptake of the synthetic peptides. These results indicated that oligo-arginine pepitde was relatively tolerant to Trp insertion in the middle position but not in the N-terminal position.
Journal
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- Journal of Tohoku Pharmaceutical University
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Journal of Tohoku Pharmaceutical University (61), 47-53, 2014-12
東北薬科大学
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Details 詳細情報について
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- CRID
- 1050001337603944576
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- NII Article ID
- 110009901319
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- NII Book ID
- AA1149311X
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- NDL BIB ID
- 026705431
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- ISSN
- 1345157X
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- Text Lang
- ja
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- Article Type
- departmental bulletin paper
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- Data Source
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- IRDB
- NDL
- CiNii Articles